Practical Considerations in the Design and Use of Immunologically Active Fibrillar Peptide Assemblies.

TitlePractical Considerations in the Design and Use of Immunologically Active Fibrillar Peptide Assemblies.
Publication TypeJournal Article
Year of Publication2018
AuthorsCM Solano, Y Wen, H Han, and JH Collier
JournalMethods in Molecular Biology (Clifton, N.J.)
Volume1777
Start Page233
Pagination233 - 248
Date Published01/2018
Abstract

The design, formulation, and immunological evaluation of self-assembling peptide materials is relatively straightforward. Indeed, one of the advantages of synthetic self-assembling peptides is that one can progress from initial concept to in vivo testing in a matter of days. However, because these materials are supramolecular, working with them is not without some practical challenges, and subtle changes in design, synthesis, handling, and formulation can affect the materials' immunogenicity. This chapter is intended to communicate some of these practical aspects of working with these materials that are not always enumerated in conventional research papers. Epitope considerations, peptide synthesis, purification, storage, nanofiber formation, quality control, immunological evaluation, and the overall phenotypic characteristics of the immune responses to be expected from these materials are discussed.

DOI10.1007/978-1-4939-7811-3_14
Short TitleMethods in Molecular Biology (Clifton, N.J.)