Epithelialization of hydrogels achieved by amine functionalization and co-culture with stromal cells.

TitleEpithelialization of hydrogels achieved by amine functionalization and co-culture with stromal cells.
Publication TypeJournal Article
Year of Publication2007
AuthorsS Rimmer, C Johnson, B Zhao, J Collier, L Gilmore, S Sabnis, P Wyman, C Sammon, NJ Fullwood, and S MacNeil
JournalBiomaterials
Volume28
Issue35
Start Page5319
Pagination5319 - 5331
Date Published12/2007
Abstract

The aim of this study was to develop a hydrogel which would be suitable for corneal cell re-epithelialization when used as a corneal implant. To achieve this, a series of hydrogels were functionalized with primary amines by post-polymerization reactions between amine compounds and glycidyl ether groups attached to the hydrogels. We report a strong correlation between the structure of the amine and the viability of stromal cells and epithelial cells cultured on these hydrogels. Subsequent co-culture of epithelial and stromal cells on the amine modified hydrogels allowed successful expansion of epithelial cells on surfaces functionalized with alkyl alpha-omega diamines with carbon chain lengths of between 3 and 6. Analysis of variance showed that corneal epithelial cells had a strong preference for surfaces functionalized by the reaction of excess 1,3 diaminopropane with units of glycidyl methacrylate compared to the reaction products of other amines (ammonia; 1,2-diaminoethane; 1,4-diaminobutane or 1,6-diaminohexane). We suggest this approach of amine functionalization combined with stromal/epithelial co-culture offers a promising new approach to achieving a secure corneal epithelium.

DOI10.1016/j.biomaterials.2007.08.028
Short TitleBiomaterials